Zadanie 4a
Identify the DNA-profiling technique used to: (i) make many copies of a DNA region; (ii) separate DNA fragments by size.
Amplifying DNA and separating fragments
Explain PCR temperature cycles, primers and thermostable polymerase, and how gel electrophoresis separates DNA fragments.
Tę umiejętność sprawdza 14 zadań wzorowanych na arkuszach IB z lat 2023–2026.
Zadania sprawdzające tę umiejętność: 14 Otwórz w wyszukiwarce z filtrami
Zadanie 6
A thermocycler follows the temperature programme shown. What is the purpose of holding the mixture at stage X? A. To expose the templates by melting hydrogen bonds B. To permit primers to anneal at the lowest temperature C. To allow the thermostable polymerase…
- Materiał graficzny
Zadanie 7
DNA fragments migrate from the wells through the gel. Which labelled migration distance corresponds to the longest fragments? A. A B. B C. C D. D
- Materiał graficzny
Zadanie 7
DNA fragments move through an agarose gel from the wells shown. Which deduction is supported? A. P travelled furthest and represents smaller DNA fragments than Q B. Q is positively charged and moves toward the negative electrode C. Q must have a lower…
- Materiał graficzny
Zadanie 8
Biological function depends on coordinated structures and recurring processes. (a) Outline the roles and regulation of progesterone during the menstrual cycle. (b) Describe the polymerase chain reaction. (c) Explain how carbon is recycled through a terrestrial…
Zadanie 2b
State a laboratory method for amplifying a chosen DNA region.
Zadanie 7
A PCR protocol repeatedly heats DNA to separate its strands. Why can a heat-stable bacterial DNA polymerase remain useful through many cycles? A. It prevents the DNA strands from separating B. It replaces the need for a template strand C. It produces the…
Zadanie 14
A laboratory prepares a DNA profile from a very small sample. During which stage is PCR used? A. Amplifying selected DNA regions to obtain many copies B. Separating fragments by their movement through a gel C. Breaking open cells to release their contents D.…
Zadanie 16
When a voltage is applied across an agarose gel, why do DNA fragments migrate towards the anode? A. Their negatively charged phosphate backbones are attracted to the positive electrode B. Their phosphate groups are positively charged at the electrophoresis pH…
Zadanie 3a
(i) State one suitable source of a person’s DNA. (ii) State how a small DNA sample can be amplified.
- Materiał graficzny
Zadanie 10
Deduce whether the gel supports successful transformation, giving a reason.
- Materiał graficzny
Zadanie 3a
State the lane number for the control lacking template DNA.
- Materiał graficzny
Zadanie 3b
Explain why the temperature changes within each cycle.
- Materiał graficzny
Zadanie 3c
Predict the result if fewer cycles are completed.
- Materiał graficzny