A paper chromatogram is shown. The distances marked were measured along the paper. What is the retardation factor, , of compound Y? A. 0.40 B. 0.60 C. 0.70 D. 1.67
Chromatography and Rf values
Explain how paper and thin-layer chromatography separate a mixture through the different attractions of components to the mobile and stationary phases, and calculate and interpret retardation factor (Rf) values from a chromatogram.
A mixture of biphenyl, , and 4-phenylphenol, , was separated by thin-layer chromatography on a polar silica plate, using the non-polar solvent cyclohexane as the mobile phase. What are the position and the retardation factor…
Each beaker used as a chromatography chamber was covered with a watch glass while the solvent rose up the paper. Explain why the chamber has to be covered for the experiment to be properly designed.
Explain why the start line must be horizontal and must be above the level of the solvent when the strip is placed in the chamber.
For the orange dye, calculate the retardation factor, , obtained in each solvent system. Dye Orange dye, system 1 (25 % ethanol) Orange dye, system 2 (75 % ethanol)
Deduce, giving a reason, which of the two dyes, orange or violet, is the more polar.
The student suspected that the ink contained more than four dyes, and that one of the spots was two dyes that had not separated. Suggest how the student could modify the chromatography experiment to test this idea.
Explain how the dyes are separated from one another in paper chromatography.
Two pigments extracted from a leaf are separated by thin-layer chromatography on a silica plate (a polar stationary phase) using a non-polar solvent as the mobile phase. Pigment X travels further up the plate than pigment Y. Which statement explains the…